PHASE I – WORK PLAN
SYNTHETIC BIOLOGY
Projects in Synthetic Biology
Decoding Aging Dynamics Using Synthetic Minimal Genome Cells and Single-Cell Tracing
This project leverages the J. Craig Venter Institute (JCVI) JCVI-syn3.0 synthetic minimal bacterial cell to study the process of aging using high-throughput experimental setups, single-cell tracing technology, and advanced mathematical modeling. This simplified system serves as an ideal model for investigating the fundamental processes of cellular aging, eliminating the complexities associated with higher organisms and providing a high-throughput platform for studying aging dynamics
Aging, a complex process affecting all living organisms, is characterized by genetic, metabolic, and environmental interactions. However, the exact molecular mechanisms driving aging remain elusive, particularly at the cellular level. By utilizing the minimal genome bacterium, which carries only the genes essential for life, we can systematically investigate the core biological functions that contribute to aging, many of which may be conserved across species. This project will allow researchers to observe how gene expression, cellular morphology, and damage accumulation change as cells age.
The Gradual Creation of Animal Models 2.0 (AM2)
This project proposes a fundamental upgrade to current animal models (referred to as animal models 1.0) by introducing synthetic and trans-organismal biological systems designed to enhance their ability to cope with aging. While animal models 1.0 have provided insights into aging by tweaking existing physiological and genetic systems, there is a perceived biological limit to their efficacy in extending lifespan or healthspan.
By introducing new genes, synthetic enzymes, evolved proteasomes, and trans-organismal organelles, animal models 2.0 (AM2) aim to breach this ceiling. These upgrades will enhance cellular processes like waste clearance, energy production, and proteostasis, offering a radical new platform to study aging and lifespan extension. This project includes several sub-projects that target key aspects of aging. Eventually, the project seeks to test these biological upgrades in larger organisms and possibly humans. By upgrading animal models to AM2, we hope to gain unprecedented insight into aging mechanisms and discover novel interventions to extend lifespan.